Green Fluorescent Protein: Properties, Applications and Protocols, 2nd EditionISBN: 978-0-471-73682-0
Hardcover
488 pages
October 2005
This is a Print-on-Demand title. It will be printed specifically to fill your order. Please allow an additional 15-20 days delivery time. The book is not returnable.
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Since the discovery of the gene for green fluorescent protein
(GFP), derived from jellyfish, this protein that emits a green glow
has initiated a revolution in molecular biosciences. With this
tool, it is now possible to visualize nearly any protein of
interest in any cell or tissue of any species. Since the
publication of the first edition, there have been tremendously
significant technological advances, including development of new
mutant variants. Proteins are now available in yellow and blue, and
Novel Fluorescent Proteins (NFPs) have expanded their utility in
developing biosensors, biological markers, and other biological
applications.
This updated, expanded new edition places emphasis on the rise of NFPs, including new chapters on NFP properties with detailed protocols, applications of GFPs and NFPs in industry research, and biosensors. This book provides a solid theoretical framework, along with detailed, practical guidance on use of GFPs and NFPs with discussion of potential pitfalls. The expert contributors provide real examples in showing how to tailor GFP/NFP to specific systems, maximize expression, and enhance detection.
This updated, expanded new edition places emphasis on the rise of NFPs, including new chapters on NFP properties with detailed protocols, applications of GFPs and NFPs in industry research, and biosensors. This book provides a solid theoretical framework, along with detailed, practical guidance on use of GFPs and NFPs with discussion of potential pitfalls. The expert contributors provide real examples in showing how to tailor GFP/NFP to specific systems, maximize expression, and enhance detection.