Green Fluorescent Protein: Properties, Applications and Protocols, 2nd Edition

Preface.

Contributors.

1. Discovery of Green Fluorescent Protein (Osamu Shimomura).

2. Photons for Reporting Molecular Events: Green Fluorescent Protein and Four Luciferase Systems (J. Woodland Hastings and James G. Morin).

3. Biochemical and Physical Properties opf Green Fluorescent Protein (William W. Ward).

4. The Three-Dimensional Structure of Green Fluorescent Protein and Its Implications for Function and Design (George N. Phillips).

5. Molecular Biology and Mutation of Green Fluorescent Protein (David A. Zacharias and Roger Y. Tsien).

6. Discovery and Properties of GFP-Like Proteins from Nonbioluminescent Anthozoa (Konstantin A. Lukyanov, Dmitry M. Chudakov, Arkady F. Fradkov, Yulii A. Labas, Mikhail V. Matz, and Sergey Lukyanov).

7. Evolution of Function and Color in GPF-Like Proteins (Mikhail V. Matz, Yulii A. Labas, and Juan Ugalde).

8. The Uses of Green Fluorescent Protein in Prokaryotes (Raphael H. Valdivia, Brendan P. Cormack, and Stanley Falkow).

9. The uses of Green Fluorescent Protein in Yeasts (Amy L. Hitchcock, Jason A. Kahana, and Pamela A. Silver).

10. Uses of GFP in Caenorhabditis Elegans (Oliver Hobert and Paula Loria).

11. Green Fluorescent Protein Applications in Drosophila (Tulle Hazelrigg and Jennifer H. Mansfield).

12.The Uses of Green Fluorescent Protein in Plants (Jim Haseloff and Kirby R. Siemering).

13. Uses of GFP in Transgenic Vertebrates (Sean Magason, Adam Amsterdam, Nancy Hopkins, and Shuo Lin).

14. The Uses of Green Fluorescent Protein in Mammalian Cells (Theresa H. Ward and Jennifer Lippincott-Schwartz).

15. Practical Considerations for Use of Reef Coral Fluorescent Proteins in Mammalian Cells: Applications in Fluorescent Microscopy and Flow Cytometry (Yu Fang, Olivier DĂry, Michael Haugwitz, Pierre Turpin, and Steven R. Kain).

16. Pharmaceutical Applications of GFP and RCFP (Nicola Bevan and Stephen Rees).

17. Reassembled GFP: Detecting Protein-Protein Interactions and Protein Expression Patterns (Thomas J. Magliery and Lynne Regan).

Methods and Protocols (Steven R. Kain).

Index.