What's Wrong With My Mouse?: Behavioral Phenotyping of Transgenic and Knockout Mice, 2nd Edition

Preface ix

Preface to the First Edition xi

Acknowledgments xiii

Chapter 1 Designer Mice 1
Scope and sourcebooks

Chapter 2 Of Unicorns and Chimeras 10
How to generate a line of transgenic or knockout mouse for behavioural phenotyping

Chapter 3 General Health 42
Give your mouse a physical

Chapter 4 Motor Functions 62
Open field, holeboard, rotarod, balance, grip, circadian activity, circling, stereotypy, ataxic gait, seizures

Chapter 5 Sensory Abilities 86
Olfaction, vision, hearing, taste, touch, nociception

Chapter 6 Learning and Memory 110
Morris swim task, spatial mazes, cued and contextual conditioning, conditioned taste aversion, conditioned eyeblink, olfactory discrimination, social recognition, passive avoidance, schedule controlled operant tasks, motor learning, attention

Chapter 7 Feeding and Drinking 164
Daily consumption, restricted access, choice tests, microstructural analysis

Chapter 8 Reproductive Behaviors 186
Sexual and parental behaviors

Chapter 9 Social Behaviors 206
Social interaction, nesting, grooming, juvenile play, aggression

Chapter 10 Emotional Behaviors: Animal Models of Psychiatric Diseases 226
Mouse models of fear, anxiety, depression, schizophrenia

Chapter 11 Reward 266
Self-administration of addictive drugs, conditioned place preference

Chapter 12 Neurodevelopment and Neurodegeneration 290
Assaying behaviors in infant, juvenile, and aged mice

Chapter 13 Putting It All Together 322
Choice of tests, order of testing, number of mice, equipment, housing and testing environment

Chapter 14 The Next Generation 344
Conditional and inducible mutations, viral vector gene delivery, RNA silencing, quantitative trait loci analysis, DNA microarrays, chemical mutagenesis, gene therapy, ethical issues

References 383

Index 503