Introduction to Proteomics

1. Introduction to Proteomics and Strategy for Proteins Identification.

2. Sample Preparation.

3. Chromatographic Separation Methods.

3.1. Liquid Chromatography Linked to Mass Spectrometry.

3.2. Multidimensional Liquid Chromatography Combined with Mass Spectrometry.

4. Electrophoretic Separation Methods.

4.1. One-Dimensional Gel Electrophoresis.

4.2. Two-Dimensional Gel Electrophoresis.

5. Capillary Electrophoresis.

6. Protein Blotting.

7. Introduction to Proteins and Peptides Mass Spectrometry.

7.1. Electrospray Ionization (ESI).

7.2. Matrix-Assisted Laser Desorption Ionization (MALDI).

7.3. Imaging Mass Spectrometry.

7.4. Fragmentation and Sequencing in Proteomics.

7.5. Protein Identification by Mass Spectrometry.

7.6. Chemical Derivatization for Sequence Analysis.

8. Protein Modifications.

9. Microfluidics.

10. Proteomics.

10.1. Quantitative Proteomics.

10.2. Structural Proteomics.

10.3. Functional Proteomics.

10.4. Clinical Proteomics.

11. Bioinformatics.

12. Appendix.

13. The Laboratory Practicals.

13.1. Polyacrylamide Gel Electrophoresis under Denaturing Conditions.

13.2. Preparation of the In-Gel Separated Proteins for Mass Spectrometry.

13.3. Bioinformatic Tools for Protein Identification.

13.4. LC-MS and LC-MS/MS - Proteomics Applications.

13.5. Enzyme Kinetics.

13.6. Internet Databases.