Advanced Genetic Analysis: Finding Meaning in a Genome

Introduction.

1. Mutation:.

Types Of Mutations.

Dominance And Recessivity.

The Genetic Utility Of Dominant And Recessive Mutants.

Summary.

Gallery Of Model Organisms.

2. Mutant Hunts:.

Why Look For New Mutants?.

Mutagenesis And Mutational Mechanisms.

What Phenotype Should You Screen (Or Select) For?.

Actually Getting Started.

Summary.

3. The Complementation Test:.

The Essence Of The Complementation Test.

Rules For Uing The Complementation Test.

How Might The Complementation Test Lie To You?.

Second-Site Non-Complementation (SSNC) (Non-Allelic Non-Complementation).

An Extension Of Second-Site Non-Complementation: Dominant Enhancers.

Summary.

4. Suppression:.

A Basic Definition Of Genetic Suppression.

Intragenic Suppression (Pseudo-Reversion).

Extragenic Suppression.

Transcriptional Suppression.

Translational Suppression.

Suppression By Post-Translational Modification.

Extragenic Suppression As A Result Of Protein–Protein Interaction.

Suppression Without Physical Interaction.

Suppression Of Dominant Mutations.

Designing Your Own Screen For Suppressor Mutations.

Summary And A Warning.

5. Determining When And Where Genes Function:.

Epistasis: Ordering Gene Function In Pathways.

Mosaic Analysis: Where Does A Given Gene Act.

Summary.

6. Genetic Fine-Structure Analysis:.

Intragenic Mapping (Then).

Intragenic Mapping (Now).

Intragenic Complementation Meets Intragenic Recombination: The Basis Of Fine-Structure Analysis.

An Example Of Fine-Structure Analysis For A Eukaryotic Gene Encoding A Multifunctional Protein.

Fine-Structure Analysis Of Genes With Complex Regulatory Elements In Eukaryotes.

Pairing-Dependent Intragenic Complementation.

Summary.

7. Meiotic Recombination:.

An Introduction To Meiosis.

Crossingover And Chiasmata: Recombination Involves The Physical Interchange Of Genetic Material And Ensures Homolog Separation.

The Classical Analysis Of Recombination.

Measuring The Frequency Of Recombination.

The Mechanism Of Recombination.

Summary.

8. Meiotic Chromosome Segregation:.

Types And Consequences Of Failed Segregation.

The Origin Of Spontaneous Non-Disjunction.

The Centromere.

Segregational Mechanisms.

Summary.

Epilogue.

References.

Partial author index.

Subject index.