Genetic Techniques for Biological Research: A Case Study Approach

SECTION I: SACCHAROMYCES CEREVISIAE AS A GENETIC RESEARCH ORGANISM

1. Saccharomyces cerevisiae As a Genetic Model Organism

2. Techniques in Cell and Molecular Biology

3. Saccharomyces Cell Structure

SECTION II: TECHNIQUES OF GENETIC ANALYSIS

4. Mutant Hunts - To select or to screen (perhaps even by brute force)

5. Complementation analysis: How many genes are involved?

6. Epistasis analysis

7. Gene isolation and analysis of multiple mutant alleles

8. Suppression Analysis

9. Enhancement and synthetic phenotypes

10. Two-hybrid analysis

11. Advanced concepts in molecular genetic analysis

12. Genomic Analysis

SECTION III: CASE STUDIES FROM THE SACCHAROMYCES GENETIC LITERATURE

Case Study I: Glucose Sensing and Signaling mechanisms in Saccharomyces

Case Study II: Secretion, Exocytosis and Vesicle Trafficking in Saccharomyces

Case Study III: The Cell Division Cycle of Saccharomyces

Case Study IV: Mating-type Pheromone Response Pathway of Saccharomyces

Index.