Genetic Techniques for Biological Research: A Case Study ApproachISBN: 978-0-471-89919-8
Hardcover
254 pages
June 2002
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SECTION I: SACCHAROMYCES CEREVISIAE AS A GENETIC RESEARCH ORGANISM.
Saccharomyces cerevisiae As a Genetic Model Organism
Techniques in Cell and Molecular Biology
Saccharomyces Cell Structure.
SECTION II: TECHNIQUES OF GENETIC ANALYSIS.
Mutant Hunts - To Select or to Screen (perhaps even by brute force).
Complementation Analysis: How many Genes are Involved?
Epistasis Analysis.
Gene Isolation and Analysis of Multiple Mutant Alleles.
Suppression Analysis.
Enhancement and Synthetic Phenotypes
Two-hybrid Analysis
Advanced Concepts in Molecular Genetic Analysis
Genomic Analysis
SECTION III: CASE STUDIES FROM THE SACCHAROMYCES GENETIC LITERATURE.
Case Study I: Glucose Sensing and signaling Mechanisms in Saccharomyces.
Case Study II: Secretion, Exocytosis and Vesicle Trafficking in Saccharomyces.
Case Study III: The Cell Division Cycle of Saccharomyces.
Case Study IV: Mating-type Pheromone Response Pathway of Saccharomyces.
Index.