Introduction to Proteomics: Principles and Applications

PREFACE.

ABOUT THE AUTHOR.

CHAPTER 1 HISTORICAL PERSPECTIVES.

1.1. Introduction to Proteomics.

1.2. Proteome and Proteomics.

1.3. Genetics of Proteins.

1.4. Molecular Biology of Genes and Proteins.

1.5. Protein Chemistry Before Proteomics.

References.

Further Reading.

CHAPTER 2 PROTEOMICS—RELATION TO GENOMICS, BIOINFORMATICS.

2.1. Genomics.

2.2. Bioinformatics and Computational Biology.

References.

Further Reading.

CHAPTER 3 METHODOLOGY FOR SEPARATION AND IDENTIFICATION OF PROTEINS AND THEIR INTERACTIONS.

3.1. Separation of Proteins Via a Multidimensional Approach.

3.2. Determination of the Primary Structure of Proteins.

3.3. Determination of the 3D Structure of a Protein.

3.4. Determination of the Amount of Proteins.

3.5. Structural and Functional Proteomics.

References.

Further Reading.

CHAPTER 4 PROTEOMICS OF PROTEIN MODIFICATIONS.

4.1. Phosphorylation and Phosphoproteomics.

4.2. Glycosylation and Glycoproteomics.

4.3. Ubiquitination and Ubiquitinomics.

4.4. Miscellaneous Modifications of Proteins.

References.

Further Reading.

CHAPTER 5 PROTEOMICS OF PROTEIN–PROTEIN INTERACTIONS/INTERACTOMES.

5.1. Protein—Protein Interactions (PPI) in Vivo.

5.2. Analysis of Protein Interactions in Vitro.

5.3. Analysis of Protein Interactions in Silico.

5.4. Synthetic Genetic Methods to Determine Protein Interactions.

5.5. Interactomes.

5.6. Evolution and Conservation of Interactomes.

5.7. Interactomes and the Complexity of Organisms: It is the Number of Interactomes that Matters in Understanding the Complexity of an Organism and not the Number of Genes.

5.8. Interaction of Proteins with Small Molecules.

References.

Further Reading.

CHAPTER 6 APPLICATIONS OF PROTEOMICS I: PROTEOMICS, HUMAN DISEASE, AND MEDICINE.

6.1. Diseasome.

6.2. Medical Proteomics.

6.3. Clinical Proteomics.

6.4. Metaproteomics and Human Health.

6.5. Proteomics in Biotechnology and Industry of Drug Production.

6.6. Metaproteomics of Microbial Fermentation.

6.7. Beef Industry.

6.8. Bioterrorism and Biodefense.

References.

Further Reading.

CHAPTER 7 PROTEOMICS—FUTURE DEVELOPMENTS.

7.1. Technical Scope of Proteomics—Beyond Protein Identification.

7.2. Scientific Scope of Proteomics—Control of Epigenesis.

7.3. Medical Scope of Proteomics.

7.4. Proteomics, Energy Production, and Bioremediation.

7.5. Proteomics and Biodefense.

References.

Further Reading.

INDEX.