Culture of Cells for Tissue Engineering

Preface vii

List of Abbreviations xi

Part I: Cell Culture

1. Basic Principles of Cell Culture 3
R. Ian Freshney

2. Mesenchymal Stem Cells for Tissue Engineering 23
Donald P. Lennon and Arnold I. Caplan

3. Human Embryonic Stem Cell Culture for Tissue Engineering 61
Shulamit Levenberg, Ali Khademhosseini, Mara Macdonald, Jason Fuller, and Robert Langer

4. Cell Sources for Cartilage Tissue Engineering 83
Brian Johnstone, Jung Yoo, and Matthew Stewart

5. Lipid-Mediated Gene Transfer for Cartilage Tissue Engineering 113
Henning Madry

Part II: Tissue Engineering

6. Tissue Engineering: Basic Considerations 131
Gordana Vunjak-Novakovic

7. Tissue Engineering of Articular Cartilage 157
Koichi Masuda and Robert L. Sah

8. Ligament Tissue Engineering 191
Jingsong Chen, Jodie Moreau, Rebecca Horan, Adam Collette, Diah Bramano, Vladimir Volloch, John Richmond, Gordana Vunjak-Novakovic, David L. Kaplan, and Gregory H. Altman

9. Cellular Photoencapsulation in Hydrogels 213
Jennifer Elisseeff, Melanie Ruffner, Tae-Gyun Kim, and Christopher Williams

10. Tissue Engineering Human Skeletal Muscle for Clinical Applications 239
Janet Shansky, Paulette Ferland, Sharon McGuire, Courtney Powell, Michael DelTatto, Martin Nackman, James Hennessey, and Herman H. Vandenburgh

11. Engineered Heart Tissue 259
Thomas Eschenhagen and Wolfgang H. Zimmermann

12. Tissue-Engineered Blood Vessels 293
Rebecca Y. Klinger and Laura E. Niklason

13. Tissue Engineering of Bone 323
Sandra Hofmann, David Kaplan, Gordana Vunjak-Novakovic, and Lorenz Meinel

14. Culture of Neuroendocrine and Neuronal Cells for Tissue Engineering 375
Peter I. Lelkes, Brian R. Unsworth, Samuel Saporta, Don F. Cameron, and Gianluca Gallo

15. Tissue Engineering of the Liver 417
Gregory H. Underhill, Jennifer Felix, Jared W. Allen, Valerie Liu Tsang, Salman R. Khetani, and Sangeeta N. Bhatia

Suppliers List 473

Glossary 483

Index 491