Protein Sequencing and Identification Using Tandem Mass Spectrometry

An Introduction to Protein Sequencing Using Tandem Mass Spectrometry.

The Primary Structure of Proteins and a Historical Overview of Protein Sequencing.

Fundamental Mass Spectrometry.

Collisionally Induced Dissociation of Protonated Peptide Ions and the Interpretation of Product Ion Spectra.

Basic Polyacrylamide Gel Electrophoresis.

The Preparation of Protein Digests for Mass Spectrometric Sequencing Experiments.

Mass Spectrometric Analysis of Tryptic Digests.

Protein Identification by Database Searching.

Sequence Analysis of Novel Proteins.

The Characterization of Post-Translationally Modified Proteins Using Tandem Mass Spectrometry.

Index.