Practical HPLC Method Development, 2nd EditionISBN: 978-0-471-00703-6
Hardcover
800 pages
March 1997
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Getting Started.
Basics of Separation.
Detection Sensitivity and Selectivity.
Sample Preparation.
The Column.
Non-Ionic Samples: Reversed- and Normal-Phase HPLC.
Ionic Samples: Reversed-Phase, Ion-Pair, and Ion-Exchange HPLC.
Gradient Elution.
Systematic Approach to the Reversed-Phase Separation of Regular Samples.
Computer-Assisted Method Development.
Biochemical Samples: Proteins, Nucleic Acids, Carbohydrates, and Related Compounds.
Chiral Separations.
Preparative HPLC Separation.
Quantitation (Including Trace Analysis).
Completing the Method: Validation and Transfer.
Appendices.
Index.
Basics of Separation.
Detection Sensitivity and Selectivity.
Sample Preparation.
The Column.
Non-Ionic Samples: Reversed- and Normal-Phase HPLC.
Ionic Samples: Reversed-Phase, Ion-Pair, and Ion-Exchange HPLC.
Gradient Elution.
Systematic Approach to the Reversed-Phase Separation of Regular Samples.
Computer-Assisted Method Development.
Biochemical Samples: Proteins, Nucleic Acids, Carbohydrates, and Related Compounds.
Chiral Separations.
Preparative HPLC Separation.
Quantitation (Including Trace Analysis).
Completing the Method: Validation and Transfer.
Appendices.
Index.