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Animal Cell Culture: Essential Methods

John M. Davis (Editor)
ISBN: 978-0-470-66658-6
Paperback
376 pages
March 2011
List Price: US $82.00
Government Price: US $56.92
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Animal Cell Culture: Essential Methods (0470666587) cover image

Contributors.

Preface.

Abbreviations.

Protocols.

1 The Cell Culture Laboratory (Sue Clarke and Janette Dillon).

2 Sterilization (Peter L. Roberts).

3 Microscopy of Living Cells (Colin Gray and Daniel Zicha).

4 Basic Techniques and Media, the Maintenance of Cell Lines, and Safety (John M. Davis).

5 Development and Optimization of Serum- and Protein-free Culture Media (Stephen F. Gorfien and David W. Jayme).

6 Cryopreservation and Banking of Cell Lines (Glyn N. Stacey, Ross Hawkins and Roland A. Fleck).

7 Primary Culture of Specific Cell Types and the Establishment of Cell Lines (Kee Woei Ng, Mohan Chothirakottu Abraham, David Tai Wei Leong, Chris Morris and Jan-Thorsten Schantz).

8 Cloning (John Clarke, Alison Porter and John M. Davis).

9 The Quality Control of Animal Cell Lines and the Prevention, Detection and Cure of Contamination (Peter Thraves and Cathy Rowe).

10 Systems for Cell Culture Scale-up (Jennifer Halsall and John M. Davis).

11 Good Laboratory Practice in the Cell Culture Laboratory (Barbara Orton).

Index.

Colour plates.

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