Platelet Proteomics: Principles, Analysis, and Applications

Foreword xiii

Preface xv

Acronyms xvii

Part I General Overview: Platelets, Sample Preparation, and Mass Spectrometry-Based Proteomics

1 Platelets and Their Role in Thrombotic and Cardiovascular Disease: The Impact of Proteomic Analysis 3
Ronald G. Stanley, Katherine L. Tucker, Natasha E. Barrett, and Jonathan M. Gibbins

2 Mass-Spectrometry-Based Proteomics: General Overview and Posttranslational Modification Analysis in the Context of Platelet Research 27
Julia Maria Burkhart and Albert Sickmann

3 Sample Preparation Variables in Platelet Proteomics for Biomarker Research 67
Maria Zellner and Rudolf Oehler

Part II Analysis of The Platelet Proteome: Global Approaches and Subproteomes

4 Two-Dimensional Gel Electrophoresis: Basic Principles and Application to Platelet Signaling Studies 89
Ángel García

5 The Platelet Membrane Proteome 111
Yotis A. Senis

6 Proteomics of Platelet Granules, Organelles, and Releasate 139
James P. McRedmond

7 The Platelet Microparticle Proteome 159
David M. Smalley

8 N-Terminal Combined Fractional Diagonal Chromatographic (COFRADIC) Analysis of the Human Platelet Proteome 185
Francis Impens, Kenny Helsens, Niklaas Colaert, Lennart Martens, Joël Vandekerckhove, and Kris Gevaert

Part III Integrated “Omics” and Application to Disease

9 Serial Analysis of Gene Expression (SAGE) For Studying the Platelet and Megakaryocyte Transcriptome 209
Michael G. Tomlinson

10 The Application of Microarray Analysis and Its Integration with Proteomics for Study of Platelet-Associated Disorders 231
Dmitri V. Gnatenko and Wadie F. Bahou

11 Platelet Functional Genomics 253
Isabelle I. Salles, Marie N. O’Connor, Daphne C. Thijssen-Timmer, Katleen Broos, and Hans Deckmyn

12 Systems Biology to Study Platelet-Related Bleeding Disorders 285
Jan-Willem N. Akkerman and Bernard De Bono

13 Platelet Proteomics in Transfusion Medicine 321
Thomas Thiele, Leif Steil, Uwe Völker, and Andreas Greinacher

14 Cardiovascular Proteomics 341
Fernando Vivanco, Fernando de la Cuesta, Maria G. Barderas, Irene Zubiri, and Gloria Álvarez-Llamas

Index 381