Human Embryonic Stem Cells: The Practical Handbook

Preface.

Section 1: Obtaining and culturing human embryonic stem cells.

1 Organization and good aseptic technique in the human embryonic stem cell laboratory.
Minal J Patel, Emma L Stephenson, and Stephen L Minger

2 Sourcing human embryonic stem cell lines.
Glyn Stacey

3 Human embryo culture for human embryonic stem cell derivation.
R. Douglas Powers and Jeannine Witmyer

4 Derivation of human embryonic stem cell lines.
Stephen Sullivan, Dieter Egli, Hidenori Akutsu, Douglas A. Melton, Kevin Eggan and Chad A. Cowan

5 Standard culture of human embryonic stem cells.
Jeremy M. Crook, Rachel Horne, and Alan Colman

6 Culture of human embryonic stem cells in chemically defined conditions.
Julie Hsu Clark and Sheng Ding

Section 2: Characterization of undifferentiated embryonic stem cells.

7 Phenotypic analysis of human embryonic stem cells.
Jonathan S. Draper,  Cheryle A. Séguin and Peter W. Andrews

8 Genetic and epigenetic analysis of human embryonic stem cells.
Laurie A. Boyer, Rudolf Jaenisch, and Maisam Mitalipova

Section 3: Manipulation of human embryonic stem cells.

9 In vivo differentiation of human embryonic stem cells.
Scott A. Noggle, Francesca M. Spagnoli, Ali H. Brivanlou

10 In vitro differentiation of human embryonic stem cells.
A. Henry Sathananthan and Alan Trounson

11 Differentiation of human embryonic stem cells into extraembryonic cell types.
Cheryle A. Séguin and Jonathan S. Draper

12 Directed differentiation of human embryonic stem cells into:
(a) Early endoderm cells.
Kenji Osafune, Alice E. Chen and Douglas A. Melton
(b) Hepatic cells.
Neta Lavon and Nissim Benvenisty
(c) Pancreatic cells.
Hiram Chipperfield

13 Directed differentiation of human embryonic stem cells into:
(a) Cardiomyocytes.
Christine Mummery, Robert Passier and Chris Denning
(b) Endothelial cells.
Carrie Soukup, Shulamit Levenberg and Ondine Cleaver
(c) Osteogenic cells.
Jeffrey M. Karp, Alborz Mahdavi, Lino S. Ferreira, Ali Khademhosseini and Robert Langer
(d) Hematopoietic in vivo repopulating cells.
Shannon McKinney-Freeman, Thorsten M. Schlaeger and George Q. Daley
(e) Lymphocytes.
Petter S. Woll and Dan S. Kaufman
(f) Myeloid cells.
Chantal Cerdan and Mickie Bhatia

14 Directed differentiation of human embryonic stem cells into:
(a) Forebrain neurons.
Emily A. Davis and Lawrence S.B. Goldstein
(b) Dopaminergic neuron.
Jan Pruszak and Ole Isacson
(c) Spinal motor neurons.
Bao-Yang Hu and Su-Chun Zhang

15  (a) Gene targeting: knock out and knock in by homologous recombination.
 Thomas P. Zwaka
(b) RNA interference in human embryonic stem cells.
M. William Lensch, Asmin Tulpule and Holm Zaehres
(c) Generation of gene reporters using bacterial artificial chromosome recombineering. Andrew J. Washkowitz and David A. Shaywitz

Afterword. Azim Surani

Index.